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Vector Laboratories
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Vector Laboratories
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Vector Laboratories
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Image Search Results
Journal: Transboundary and emerging diseases
Article Title: Evidence for overwintering and autochthonous transmission of Usutu virus to wild birds following its redetection in the United Kingdom.
doi: 10.1111/tbed.14738
Figure Lengend Snippet: FIGURE 3 Detection of flavivirus NS1 antigen and colocalization with histopathology in Eurasian blackbird (Turdus merula) tissues: (a) cerebrum, (b) cerebellum, (c) liver and (d) kidney, from the Usutu virus RT-PCR positive bird submitted in 2021 from Greater London, UK, using immunohistochemistry on formalin-fixed paraffin-embedded tissue sections at ×200 magnification. Strong immunolabelling is observed in all assessed tissues, indicated by darker red/brown areas. Neuronal necrosis (arrowhead) can be detected in areas colocalized with virus antigen positive neurons and capillaries (shown in insets) of the forebrain (a) and the molecular layer of the cerebellum (b). Antibody used: anti-DENV NS1. Images taken at ×200 magnification
Article Snippet: Slides were blocked with normal goat serum (1/66 dilution; Vector Laboratories, USA) followed by incubation with
Techniques: Histopathology, Virus, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Formalin-fixed Paraffin-Embedded